Journal: Frontiers in Plant Science

Article Title: Assessment of nematicidal and plant growth-promoting effects of Burkholderia sp. JB-2 in root-knot nematode-infested soil

doi: 10.3389/fpls.2023.1216031

Figure Lengend Snippet: Effect of Burkholderia sp. JB-2 cell-free filtrates on ROS accumulation in Meloidogyne incognita J2. The 50 J2 of M. incognita were treated with 10% cell-free filtrates of JB-2 and were subsequently incubated for 24h at 25°C. 50 mM H 2 O 2 was used as the positive control and LB broth was used as the negative control. Quantitative analysis of vacuolization and ROS accumulation in M . incognita using a fluorescence probe, H 2 DCF-DA (A) . Left-hand panels are bright-field images; right-hand panels are fluorescent images of the nematodes captured by the fluorescence stereo microscope equipped with a TUCSEN Dhyana 400 DC digital camera. Qualitative analysis of ROS accumulation in M . incognita J2 using a fluorescence spectrophotometer by recording the fluorescence intensity at λex 485 nm and λem 535 nm (B) . The experiment was performed in triplicate under the same conditions. Different letters above the error bars indicate significant differences by Scheffé’s test ( P < 0.05).

Article Snippet: Images were captured using a fluorescence stereo microscope (Olympus SZX16, Tokyo, Japan) equipped with a TUCSEN Dhyana 400 DC digital camera (Olympus).

Techniques: Incubation, Positive Control, Negative Control, Fluorescence, Microscopy, Spectrophotometry